Coding

Part:BBa_K2818000:Design

Designed by: Danny Teo Shun Xiang   Group: iGEM18_NTU-Singapore   (2018-10-07)


∆RuvCIII-2 ∆HNH ∆REC2 4QC_HNHpK Sp-dCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 251
    Illegal BglII site found at 804
    Illegal BamHI site found at 1622
    Illegal XhoI site found at 2608
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1996
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2142
    Illegal BsaI site found at 2804
    Illegal BsaI.rc site found at 1027


Design Notes

The further addition of cationic mutations as well as fusion of short cationic peptides to improve DNA binding affinity of truncated Sp-dCas9.


Source

Streptococcus pyogenes, sequence optimized for homo sapien codon preference.

References